Comparison of euro-collins solution, low-potassium dextran solution containing glucose, and ET-kyoto solution for lung preservation in an extracorporeal rat lung perfusion model.

We have recently developed a modified ET-Kyoto solution by adding N-acetylcysteine, nitroglycerin, and dibutyryl cyclic adenosine monophosphate to the previously reported ETKyoto solution. The purpose of this study was to compare the new ET-Kyoto solution with the regular Euro-Collins solution and a low-potassium dextran solution containing glucose (Perfadex®) in an isolated rat lung perfusion model. Rat lung blocks were preserved with ET-Kyoto solution, with Euro-Collins solution, or with Perfadex for 4 h. Arterial oxygen tension, shunt fraction, peak inspiratory pressure, mean pulmonary arterial pressure, and pulmonary vascular resistance were assessed up to 50 min after reperfusion. ET-Kyoto solution provided a significantly better lung function after reperfusion than Euro-Collins solution and Perfadex, while Perfadex was also superior to the Euro-Collins solution. We conclude that lung preservation with ET-Kyoto solution is significantly superior to Euro-Collins solution and to Perfadex in an isolated rat lung perfusion model. OOOOOOOOOOOOOOOOO Received: September 12, 1997 Accepted: December 16, 1997 Dr. T. Bando Department of Thoracic Surgery, Chest Disease Research Institute Kyoto University, Shogoin Kawahara-cho 53, Sakyo-ku Kyoto 606 (Japan) Tel. +81 75 751 3880, Fax +81 75 751 4647 ABC Fax + 41 61 306 12 34 E-Mail [email protected] www.karger.com © 1998 S. Karger AG, Basel 0014–312X/98/0305–0297$15.00/0 Accessible online at: http://BioMedNet.com/karger Introduction Current clinical lung preservation with Euro-Collins (EC) or University of Wisconsin solution provides satisfactory graft function. The tolerable ischemic time, however, is still limited to approximately 7 h [1]. Strategies to increase the ischemic tolerance and as a consequence the supply of transplantable lungs are, therefore, needed. We already reported